Data underlying the publication: Species sorting shapes the divergence of a natural bacterial community with repeatable functionality during propagation with alternative substrates
DOI: 10.4121/82fa6380-c40c-4c40-b5ec-933b0253e6ce
Datacite citation style
Dataset
This data is derived from a field based adaptive evolution experiment. Here, we explored a process of species sorting and its consequence on community-level functionality using mabisi, a traditional Zambian fermented milk, by exposing a common mabisi microbial community to five distinct substrates and propagating replicate communities at three farm sites in rural Zambia. The substrates - raw bovine milk (control), low-fat milk, full-cream milk, and the infant formulas F100 and S26 – represented novel environments, were propagated at a fixed dilution and static conditions to generate ~ 66 generations. Microbial community composition was profiled through 16S rDNA sequencing, while community-level functioning was assessed through volatile organic compound profiling by GC-MS, pH changes with a pH meter, and consistency analysis using an Adam's consistometer.
History
- 2025-08-14 first online
- 2025-08-21 published, posted
Publisher
4TU.ResearchDataFormat
pdf, excel, qmd, fasta, nwkFunding
- Wageningen Global Sustainability Programme (formerly INREF) (grant code not applicable) Sijmen Schoustra
Organizations
Laboratory of Genetics, Plant Science Group, Wageningen University and Research;Department of Biomedical Sciences, National Health Research and Training Institute (formerly Tropical Diseases research Centre), Zambia;
Department of Food Science and Nutrition, The University of Zambia, Zambia
DATA
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ReadMeFile.pdf - 17,635 bytesMD5:
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16SAnalysis_FieldExperiment.qmd - 1,768,772 bytesMD5:
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ASV-dna-sequences.fasta - 20,597 bytesMD5:
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ComFunctionAnalysis_subFieldexp.qmd - 14,564 bytesMD5:
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raw_substrate_GCMS_UptoC10LEfSe.xlsx - 31,076 bytesMD5:
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Shepherd_F038_pH&Consistency_ManuscriptLEfSe.xlsx - 272,597 bytesMD5:
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sn_F038_GCMS_manuscriptLEfSE.xlsx - 2,552,670 bytesMD5:
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sn_F038_seqIDLEfSe.xlsx - 153,799 bytesMD5:
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