cff-version: 1.2.0 abstract: "

This data is derived from a field based adaptive evolution experiment. Here, we explored a process of species sorting and its consequence on community-level functionality using mabisi, a traditional Zambian fermented milk, by exposing a common mabisi microbial community to five distinct substrates and propagating replicate communities at three farm sites in rural Zambia. The substrates - raw bovine milk (control), low-fat milk, full-cream milk, and the infant formulas F100 and S26 – represented novel environments, were propagated at a fixed dilution and static conditions to generate ~ 66 generations. Microbial community composition was profiled through 16S rDNA sequencing, while community-level functioning was assessed through volatile organic compound profiling by GC-MS, pH changes with a pH meter, and consistency analysis using an Adam's consistometer.

" authors: - family-names: Nehanda given-names: Shepherd orcid: "https://orcid.org/0009-0005-4297-8829" - family-names: Alekseeva given-names: Anna orcid: "https://orcid.org/0000-0003-0164-0539" - family-names: van Mastrigt given-names: Oscar orcid: "https://orcid.org/0000-0001-6576-910X" - family-names: Chileshe given-names: Justin orcid: "https://orcid.org/0000-0003-4134-2851" - family-names: Zwaan given-names: Bas J. orcid: "https://orcid.org/0000-0002-8221-4998" - family-names: Smid given-names: Eddy J. orcid: "https://orcid.org/0000-0002-6687-5083" - family-names: Schoustra given-names: Sijmen E. orcid: "https://orcid.org/0000-0001-7843-5539" title: "Data underlying the publication: Species sorting shapes the divergence of a natural bacterial community with repeatable functionality during propagation with alternative substrates" keywords: version: 2 identifiers: - type: doi value: license: CC BY 4.0 date-released: 2025-08-21