%0 Generic
%A Nehanda, Shepherd
%A Alekseeva, Anna
%A van Mastrigt, Oscar
%A Chileshe, Justin
%A Zwaan, Bas J.
%A Smid, Eddy J.
%A Schoustra, Sijmen E.
%D 2025
%T Data underlying the publication: Species sorting shapes the divergence of a natural bacterial community with repeatable functionality during propagation with alternative substrates
%U 
%R 
%K microbial community diversity
%K adaptation
%K selection
%K infant formula
%K lactic acid bacteria
%X <p>This data is derived from a field based adaptive evolution experiment. Here, we explored a process of species sorting and its consequence on community-level functionality using mabisi, a traditional Zambian fermented milk, by exposing a common mabisi microbial community to five distinct substrates and propagating replicate communities at three farm sites in rural Zambia. The substrates - raw bovine milk (control), low-fat milk, full-cream milk, and the infant formulas F100 and S26 – represented novel environments, were propagated at a fixed dilution and static conditions to generate ~ 66 generations. Microbial community composition was profiled through 16S rDNA sequencing, while community-level functioning was assessed through volatile organic compound profiling by GC-MS, pH changes with a pH meter, and consistency analysis using an Adam&#x27;s consistometer. </p>
%I 4TU.ResearchData