Data accompanying the publication: Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo assays
Datacite citation style:
Tschirpke, Sophie; Daalman, Werner; Laan, Liedewij (2024): Data accompanying the publication: Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo assays. Version 2. 4TU.ResearchData. dataset. https://doi.org/10.4121/ac196f25-1c20-4c0c-a0b9-f01cd3fadc45.v2
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Dataset
The publication describes how to use and analyse data generated with the GTPase assay 'GTPase-Glo assay' by Promega. The data set exemplifies the protocol steps (input, analysis code, output files) described in the accompanying publication, using 2 concrete examples. The data set contains the following files: (1) assay data (luminescence/ amount of remaining GTP for various time points and GTPase enzyme concentrations) for Cdc42 and Ras. (2) a python scirpt used to reformat the data. (3) matlab code for data analysis. (4) output data (rates and plots) that the matlab code generates.
History
- 2023-11-27 first online
- 2024-01-22 published, posted
Publisher
4TU.ResearchDataFormat
xlsx, mat, m, ipynb, tiff, pdfAssociated peer-reviewed publication
Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo assaysReferences
Funding
- funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (grant agreement 758132) (grant code 758132) European Research Council
- funding from the Netherlands Organization for Scientific Research (Nederlandse Organisatie voor Wetenschappelijk Onderzoek) through a Vidi grant (016.Vidi.171.060) (grant code 016.Vidi.171.060) Netherlands Organization for Scientific Research
Organizations
TU Delft, Faculty of Applied Sciences, Department of Bionanoscience, Liedewij Laan LabDATA
Files (5)
- 4,395 bytesMD5:
a5af2e61c58192a5a442979cbeb3936cREADME.txt - 45,012 bytesMD5:
f2fd2f1e9e74509746fcdb09325fc495example1.xlsx - 25,081 bytesMD5:
d5557fd27963f8aaa260c2fce540867aexample2-matlab.xlsx - 22,233,022 bytesMD5:
e2768443de1630cc505dcd99f1ca32d2matlab.zip - 17,742 bytesMD5:
a31b85e4d9b98022dd05fbfb326809c1Ras_example.ipynb -
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