%0 Generic %A Tschirpke, Sophie %A Daalman, Werner %A Laan, Liedewij %D 2024 %T Data accompanying the publication: Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo assays %U %R 10.4121/ac196f25-1c20-4c0c-a0b9-f01cd3fadc45.v2 %K Cdc42 %K Ras %K GTPase %K GTPase activity %K rates %K in vitro %X
The publication describes how to use and analyse data generated with the GTPase assay 'GTPase-Glo assay' by Promega. The data set exemplifies the protocol steps (input, analysis code, output files) described in the accompanying publication, using 2 concrete examples. The data set contains the following files: (1) assay data (luminescence/ amount of remaining GTP for various time points and GTPase enzyme concentrations) for Cdc42 and Ras. (2) a python scirpt used to reformat the data. (3) matlab code for data analysis. (4) output data (rates and plots) that the matlab code generates.
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