Data underlying the chapter: Discovery and synthetic applications of a NAD(P)H-dependent reductive aminase from Rhodococcus erythropolis
doi: 10.4121/6fda9cb1-beb8-4985-a210-a9625eca8899
This dataset contains data collected during experiments at Delft University of Technology, as Chapter 5 of the dissertation written by Ewald Jongkind. The aim of this work was to find and characterize reductive aminases from ammonia, where we focussed on one enzyme from Rhodococcus with the highest activity. This work was done by searching for new enzymes with EnzymeMiner, express and purify active enzymes and characterize these enzymes. Dataset contains output data from our enzyme search in Excel, images of enzyme purifications, conversions and activities determined with UV-VIS (calculated in Excel), and NMR-spectra of synthesized products. This rough data was uploaded in combination with a draft of the Supporting Information to be submitted for publication.
- 2024-08-02 first online, published, posted
- ERC European Union's Horizon 2020 research and innovation programme no. 949910 (grant code 949910)
University of York, Department of Chemistry, York Structural Laboratory
DATA
- 2,479 bytesMD5:
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README5.txt - 5,758,713 bytesMD5:
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2024-07-26 Supporting information Jongkind et al..docx - 1,649,577 bytesMD5:
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Ch5E01 SDS-PAGEs bacterial RedAms.pptx - 2,502,276 bytesMD5:
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CH5E02 RedAm SDS PAGE noc ryth rop roc.pptx - 418,391 bytesMD5:
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Ch5E03 EnzymeMiner Selection Table 1.xlsx - 39,863 bytesMD5:
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Ch5E04 2023-08-10 cfe activity assay working redams.xlsx - 27,482 bytesMD5:
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Ch5E05 2024-05-22 screening Bacterial RedAms Pih and Shy_Kar_BacRedAm.xlsx - 50,566 bytesMD5:
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Ch5E06 2023-03-06 RytRedAm activity in different pH.xlsx - 715,702 bytesMD5:
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Ch5E07 2024-02-12 rytredam cyclohexanone screening.xlsx - 34,110 bytesMD5:
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Ch5E08 2023-02-21 RytRedAm temperature profile.xlsx - 66,831 bytesMD5:
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Ch5E09 2023-07-18 nadph michaelis mentencurve.xlsx - 5,816 bytesMD5:
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CH5E09 NADPH kinetics in IGOR.pxp - 62,775 bytesMD5:
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Ch5E10 2023-05-22 NADH michaelis mentencurve.xlsx - 11,305 bytesMD5:
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Ch5E10 NADH kinetics in IGOR.pxp - 424,087 bytesMD5:
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Ch5E11 2023-07-21 conversions with nad or nadp 1 hour.xlsx - 460,339 bytesMD5:
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Ch5E12 2023-04-24 time plot cyclohexanone hexanal 25 mm amine.xlsx - 65,846 bytesMD5:
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Ch5E13 2022-07-13 activity loss redam over time.xlsx - 1,917,274 bytesMD5:
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Ch5E14 2023-10-27 overview RytRedAm screening.xlsx - 6,883,988 bytesMD5:
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Ch5E15 NMR spectra.zip -
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