Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''

DOI:10.4121/21494520.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/21494520

Datacite citation style

Krzysica, Paulina; Verhoog, Loes; de Vries, Sonja; Smits, Coen; Savelkoul, H.F.J. (Huub) et. al. (2022): Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/21494520.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines.

To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.

History

  • 2022-12-09 first online, published, posted

Publisher

4TU.ResearchData

Format

xlsx

Organizations

Cell Biology and Immunology, Wageningen University & Research
Animal Nutrition, Wageningen University & Research

DATA

Files (10)