Dataset underlying publication: CarboTag: a modular approach for live and functional imaging of plant cell walls

DOI:10.4121/3464fadd-ccb8-4a6c-9463-e3014bcdf984.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/3464fadd-ccb8-4a6c-9463-e3014bcdf984

Datacite citation style

Besten, Maarten; Hendriksz, Milan; Michels, Lucile; Charrier, Benedicte; Smakowska-Luzan, Elwira et. al. (2024): Dataset underlying publication: CarboTag: a modular approach for live and functional imaging of plant cell walls. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/3464fadd-ccb8-4a6c-9463-e3014bcdf984.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Wageningen University and Research logo

Geolocation

Wageningen University and research
lat (N): 51.984
lon (E): 5.6615
view on openstreetmap

Time coverage

2022-2024

Licence

CC BY 4.0

Interoperability

Plant cells are contained inside a rigid network of cell walls. Cell walls are highly dynamic structures that act both as a structural material and as a hub for a wide range of signaling processes. Despite its crucial role in all aspects of the plant life cycle, live dynamical imaging of the cell wall and its functional properties has remained challenging. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag relies on a small molecular motif, a pyridine boronic acid, that targets its cargo to the cell wall, is non-toxic and ensures rapid tissue permeation. We designed a suite of cell wall imaging probes based on CarboTag in any desired color for multiplexing. Moreover, we created new functional reporters for live quantitative imaging of key cell wall features: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag opens the way to dynamical and quantitative mapping of cell wall responses at subcellular resolution.

History

  • 2024-12-11 first online, published, posted

Publisher

4TU.ResearchData

Funding

  • Joris Sprakel and Maarten Besten are funded by the European Research Council (ERC) project CoG-CATCH (grant code CoG-CATCH) European Research Council (ERC)

Organizations

Laboratory of Biochemistry, Wageningen University and Research;
Institute of Functional Genomics in Lyon (IGFL)

DATA

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