Data underlying the chapter: Discovery and synthetic applications of a NAD(P)H-dependent reductive aminase from Rhodococcus erythropolis

Authors: Ewald P.J. Jongkind, Jack Domenech, Arthur Govers, Marcel van den Broek, Jean-Marc Daran, Gideon Grogan, Caroline E. Paul

Short description: This dataset contains data collected during experiments at Delft University of Technology, as Chapter 5 of the dissertation written by Ewald Jongkind. Document represents Supporting information of manuscript to be submitted for publication.

Contact person: Caroline Paul, c.e.paul@tudelft.nl

Delft University of Technology – Faculty of Applied Sciences
Van der Maasweg 9 , 2629 HZ, Delft, The Netherlands

***Purpose of the test campaign***
The purpose of these experiments was to search and characterize RedAms from bacterial databases.
The data in this dataset was collected in the Biocatalysis Laboratory of the Delft University of Technology - Faculty of Applied Sciences, between September 2019 and September 2024.

**Test equipment***
Test equipment information could be found in the supplementary information of the chapter.
***Description of the data in this data set***

Corresponding methods and conditions could be found in the supporting information of the chapter.

Document(s):
2024-07-26 Jongkind et al. ESI EPJJ.docx
Ch5E01 SDS-PAGEs bacterial RedAms
CH5E02 RedAm SDS PAGE noc ryth rop roc
Ch5E03 EnzymeMiner Selectie Table 1 (output from EnzymeMiner database search)
Ch5E04 2023-08-10 cfe activity assay working redams (measured with UV-VIS)
Ch5E05 2024-05-22 screening Bacterial RedAms Pih and Shy_Kar_BacRedAm (measured with UV-VIS)
Ch5E06 2023-03-06 RytRedAm activity in different pH (measured with UV-VIS)
Ch5E07 2024-02-12 rytredam cyclohexanone screening (measured with GC-FID)
Ch5E08 2023-02-21 RytRedAm temperature profile (measured with UV-VIS)
Ch5E09 2023-07-18 nadph michaelis mentencurve (measured with UV-VIS)
CH5E09 NADPH kinetics in IGOR (made with Excel sheet above)
Ch5E10 2023-05-22 NADH michaelis mentencurve (measured with UV-VIS)
Ch5E10 NADH kinetics in IGOR (made with Excel sheet above)
Ch5E11 2023-07-21 conversions with nad or nadp 1 hour (measured with GC-FID)
Ch5E12 2023-04-24 time plot cyclohexanone hexanal 25 mm amine (measured with GC-FID)
Ch5E13 2022-07-13 activity loss redam over time (measured with UV-VIS)
Ch5E14 2023-10-27 overview RytRedAm screening (measured with GC-FID)
Ch5E15 NMR spectra