Title of the dataset:
Data underlying the publication: Lactate metabolism and microbiome composition are affected by nitrogen gas supply in continuous lactate-based chain elongation

Authors:
Carlos A. Contreras-Dávila, Arielle Ali, Cees N.J. Buisman and David P.B.T.B. Strik

Contact information:
Dr. D.P.B.T.B. Strik
david.strik@wur.nl
Environmental Technology
Wageningen University & Research
Axis-Z, Bornse Weilanden 9, 6708 WG Wageningen (the Netherlands)

Reference to the article:
Lactate metabolism and microbiome composition are affected by nitrogen gas supply in continuous lactate-based chain elongation
Carlos A. Contreras-Dávila, Arielle Ali, Cees N.J. Buisman and David P.B.T.B. Strik
Fermentation (2021)

List of files in the dataset:
- Calculated gas production.csv
Description: calculated production of hydrogen, carbon dioxide and methane based on their concentrations, N2 gas inflow and total gas outflow. Gas production during gas addition phases was corrected for under/overestimation based on N2 balances.
- Calculated HRT.csv
Description: calculated hydraulic retention time (HRT) based on inflows of medium and acid for pH control and liquid volume of the reactor.
- Carbon and electron balances.csv
Description: calculated carbon and electron balances based on carbon/electrons flowing in and out of the reactor in the gas and liquid phases. Carbon and electron equivalents for each molecule are given in Constants.csv
- Constants.csv
Description: molar weight, carbon atoms, electron equivalents and pKa values for every molecule measured.
- GC and HPLC raw data.csv
Description: raw values of concentrations measured with GC and HPLC for liquid and gas metabolites in the off gas and effluent, respectively.
- Influent effluent concentrations.csv
Description: concentrations measured for liquid metabolites in the influent and effluent.
- Productivities electron.csv
Description: electron-based productivities calculated as the difference between electron flowing in and out of the reactor in the off gas and liquid phases.
- Raw data checkup.csv
Description: journal with values from regular monitoring of the reactor.
- TSS and VSS.csv
Description: measurements of total (TSS) and volatile (VSS) suspended solids in the reactor.

Methods:
For reaction operation, quantification and calculation methods, see paper methods and supporting information

Licence: CC-BY
