README

*** Look-up table for assessment of iron content in Magtrace®  samples based on Sentimag®   ***
Author: S. Salamzadeh

Contact information: s.salamzadeh@utwente.nl 
Magnetic Detection & Imaging group
Technohal, University of Twente
Enschede, Netherlands

*** General introduction ***
Sentimag® device (Endomagnetics Ltd. UK) is employed as a handheld magnetic tool to identify the initial draining lymph node(s)
originating from a primary tumour, commonly known as the sentinel lymph node (SLN).
The evaluation is primarily qualitative and does not provide information regarding the quantity of iron tracer present in the SLN.

*** Purpose of the experiment ***
A look-up table (LUT) dataset has been created exclusively for the Sentimag® handheld probe (Endomagnetics ltd. UK).
This LUT serves the purpose of quantifying the accumulation of Magtrace magnetic tracer within SLN through the utilisation of magnetic readout counts.

*** Test equipment ***
All tests were performed with the Sentimag® (Endomagnetics Ltd. UK).
15 Magtrace® samples, each with a total volume of 35µL were used in that test.
The iron content of these samples ranged from of 0 to 504 µg, encompassing the following amounts:
 0, 1.12µg, 5.04µg, 10.08µg, 28µg, 50.4µg, 100.8µg, 120µg, 140µg, 182µg, 210µg, 252µg, 280µg, 420µg, and 504µg.
Samples were diluted with 0.9%Salin.The sample preparation is included in attached excel file in "Sample" sheet.
For the test the glass tube with an outer diameter of 8 mm and an inner diameter of 6.5 mm was used and
the position of glass tubesfixed in center of the probe with a styrofoam cubic.

****Data***
The LUT is generated by applying first order polynomial fit on measured data and data is included in Excel file "LUT-Sentimag-Magtrace.xlsx".
The "Fitted data.tif" demonstates the measured and generated LUT data for three different settings of Sentimag®.