
Title of the dataset:
R code and data for paper: Upgrading ammonia-nitrogen from manure into body proteins in black soldier fly larvae

Authors:
Alejandro Parodi [a]		ORCID 0000-0003-1351-138X
Qifa Yao [a]
Walter J.J. Gerrits [b]		ORCID 0000-0003-0494-9259
Maryia Mishyna [c]		ORCID 0000-0001-9079-8156
Catriona M.M. Lakemond [c]	ORCID 0000-0002-9345-7733
Dennis G.A.B. Oonincx [b]	ORCID 0000-0002-6728-4859
Joop J.A. Van Loon [d]		ORCID 0000-0002-4260-0501

[a] Animal Production Systems group, Wageningen University & Research, P.O. Box 338, 6700 AH Wageningen, the Netherlands
[b] Animal Nutrition group, Wageningen University & Research, P.O. Box 338, 6700 AH Wageningen, the Netherlands
[c] Food Quality & Design Group, Wageningen University & Research, 6700 AA, Wageningen, the Netherlands
[d] Laboratory of Entomology, Wageningen University & Research, P.O. Box 16, 6700 AA Wageningen, the Netherlands


Related publication:
Upgrading ammonia-nitrogen from manure into body proteins in black soldier fly larvae. Resources, Conservation and Recycling

Description:
The aim of the study was to quantify the incorporation of ammonia (NH3)-nitrogen into black soldier fly body mass. For that we performed an experiment in which black soldier fly was reared in pig manure enriched with the isotope 15N in NH3. Larval samples were then analyzed for the presence of 15N. The outcome of this study contributes to quantify the potential of BSFL for manure management and as a circular protein source.

Keywords
Black soldier fly, Hermetia illucens, ammonia, manure, manure management


This dataset contains the following files:

1. 15N_isotope_BSF.R
   Contains R code for analysis and visualizations

2. in_larvae_weight.csv
   Number of larvae and weight at the beginning and end of the experiment.

3. in_balance.csv
   Contains data to build the nutrient balances (larval yields, DM, N and 15N content in each sample)

4. in_protein_extraction.csv
   Contains the data to build the N and 15N balance after protein extraction.

5. in_ammonia.csv
   Contains data on ammonia-N content for manure and residual material


Explanation of columns in each dataset:

in_larvae_weight.csv

#[Sample] -> Type of sample (manure, starter, larvae or residues)
#[Treatment] -> Specifies the treatment (Enriched, Enriched_Digesta and Control) for the data shown in the row
#[Replicate] -> Number of replicate (R1-R5)
#[Larvae_added] -> Number of larvae added at the start of the experiment
#[Larvae_found] -> Number of larvae found when larvae were removed from manure
#[Total_fresh_before_NFreediet] -> Weight in grams of the larvae when were removed from manure
#[Total_fresh] -> Weight in grams of the larvae when were removed from the Nitrogen Free diet. 
                  #For the treatment Enriched_Digesta, this value is equal to the previous column 
                  #given that those larvae were not put in the N free diet.


in_balance.csv

#[Type] -> Specifies if the data show in the row is for an input or output
#[Sample] -> Type of sample (manure, starter, larvae or residual_material)
#[Treatment] -> Specifies the treatment (Enriched, Enriched_Digesta and Control) for the data shown in the row
#[Replicate] -> Number of replicate (R1-R5)
#[Total_fresh] -> Weight in grams of the larvae when were removed from the Nitrogen Free diet. For the treatment Enriched_Non_NFree, this value is equal to the previous column given that those larvae were not put in the N free diet.
#[DM] -> DM content (%) af larvae
#[N] -> Total nitrogen content (%) of DM larvae
#[N15] -> 15N atomic percentage (%) in DM larvae

in_protein_extraction.csv

#[Type] -> Specifies if the data show in the row is for an input or output
#[Sample] -> Type of sample (insoluble nitrogen fraction, soluble dialysed nitrogen fraction)
#[Treatment] -> Specifies the treatment (Enriched, Enriched_Digesta and Control) for the data shown in the row
#[Replicate] -> Number of replicate (R1-R5)
#[Yield] - Percentage (%) of DM recovered after the extraction of the insoluble and soluble dialysed N fractions
#[N] -> Total nitrogen content (%) of each N DM fraction
#[N15] -> 15N atomic percentage (%) of each N fraction

in_ammonia.csv

#[Type] -> Specifies if the data show in the row is for an input or output
#[Sample] -> Type of sample (manure and residual material)
#[Treatment] -> Specifies the treatment (Enriched, Enriched_Digesta and Control)
#[Replicate] -> Number of replicate (R1-R5)
#[nutrient] -> Only contains data for ammonia nitrogen (N-NH3)
#[value] -> ammonia-nitrogen in 100 g DM sample

...

Methods, materials and software:
Sofware: 
R version 4.0.3 (2020-10-10)
RStudio version 1.3.1093
...

This dataset is published under the CC BY-SA (Attribution ShareAlike) license.