TY - DATA T1 - Software for "Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body" PY - 2025/10/01 AU - Enya S. Berrevoets AU - Laurell F. Kessler AU - Ashwin Balakrishnan AU - Ellen Kazumi Okuda AU - Michaela Müller-McNicoll AU - Mike Heilemann AU - Bernd Rieger AU - Sjoerd Stallinga UR - DO - 10.4121/e0d424e4-5833-4729-a5a0-48ce94ed3f16.v1 KW - Smart Microscopy KW - Super-Resolution Microscopy KW - STED KW - Morphological Sorting KW - Spherical Harmonics KW - Polarization Analysis KW - Paraspeckle KW - Architectural RNA KW - NEAT1 N2 -

This software repository contains the code accompanying the paper "Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body". The repository includes a python algorithm to automatically detect and image paraspeckles using an Abberior STED microscope, as well as Matlab code to rotationally and translationally align the resulting paraspeckle images and perform shape and polarity analyses on them.

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