TY - DATA T1 - Data underlying the manuscript: Invasive cancer cells soften collagen networks and disrupt stress stiffening via volume exclusion, contractility and adhesion PY - 2025/04/15 AU - Irène Nagle AU - Margherita Tavasso AU - Ankur Deep Bordoloi AU - Iain Muntz AU - G.H. (Gijsje) Koenderink AU - Pouyan Boukany UR - DO - 10.4121/5dd62a23-7f71-4674-b232-7c8316ce5492.v1 KW - Cell-matrix interactions KW - Shear rheology KW - Actin cytoskeleton KW - Tissue mechanics KW - Active matter KW - Rheo-confocal microscopy N2 -

The research investigates how local cell–ECM interactions regulate the global mechanics of collagen networks in a biomimetic tissue model composed of type I fibrillar collagen with embedded cancer cells (volume fractions: 0.4%–20%). Experiments compare the behavior of human dermal fibroblasts and MDA-MB-231 breast cancer cells, which are known to actively remodel collagen networks. Collagen polymerization and network mechanics were characterized using bulk shear rheology. A custom-built rheo-confocal microscope was used to visualize real-time local remodeling of collagen during polymerization. All raw data, processed results, and MATLAB/python scripts for data analysis and figure generation are included.

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