The aim of this publication is to prepare polyelectrolyte complex membranes incorporated with lysozyme through a one-step and mild pH shift aqueous phase separation (APS) approach. The effects of lysozyme addition and casting solution pH on the membrane properties were studied. The biocatalytic membranes exhibited temperature dependent enzymatic activity. Moreover, the biocatalytic membranes demonstrate desirable enzymatic stability, maintaining 60% activity even after 60 days of storage. This study validates the potential of the water-based APS process as a straightforward approach for integrating enzymes into responsive biocatalytic membranes.
The dataset contains the data presented in the publication: dynamic viscosity of the casting solutions, SEM images, pure water permeability, and the enzymatic activity data of all the membranes.
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