TY - DATA T1 - Test data for Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view PY - 2024/12/03 AU - Isabel Droste AU - Erik Schuitema AU - Sajjad Khan AU - Stijn Heldens AU - Ben van Werkhoven AU - Keith A. Lidke AU - Sjoerd Stallinga AU - Bernd Rieger UR - DO - 10.4121/311fa522-2ce5-47f6-8674-10e001f7e909.v1 KW - microtubules KW - test data KW - Single molecule localization microscopy N2 - <p>Test data for the software demo of the manuscript 'Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view'. <span style="background-color: rgb(255, 250, 234);">This dataset contains the first 800 frames from the 3D microtuble dataset from Figure 2 of the manuscript. It is a test dataset for the Software Demo accompanying the manuscript.</span></p><p><br></p><p><span style="background-color: rgb(255, 250, 234);">It concerns raw</span> data for Single Molecule Localization Microscopy of microtubules of HeLa cells over a large 97 x 97 μm field of view. The images were acquired with 2 cylindrical lenses at the Fourier plane of the microscope to introduce astigmatism. The data was acquired in order to demonstrate the estimation of field dependent aberrations from single molecule localization.</p> ER -