cff-version: 1.2.0
abstract: "<p>This software repository contains the code accompanying the paper "Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body". The repository includes a python algorithm to automatically detect and image paraspeckles using an Abberior STED microscope, as well as Matlab code to rotationally and translationally align the resulting paraspeckle images and perform shape and polarity analyses on them. </p>"
authors:
  - family-names: Berrevoets
    given-names: Enya S.
    orcid: "https://orcid.org/0009-0003-1097-9697"
  - family-names: Kessler
    given-names: Laurell F.
  - family-names: Balakrishnan
    given-names: Ashwin
    orcid: "https://orcid.org/0000-0003-4059-8345"
  - family-names: Okuda
    given-names: Ellen Kazumi
  - family-names: Müller-McNicoll
    given-names: Michaela
    orcid: "https://orcid.org/0000-0002-7174-8310"
  - family-names: Heilemann
    given-names: Mike
    orcid: "https://orcid.org/0000-0002-9821-3578"
  - family-names: Rieger
    given-names: Bernd
    orcid: "https://orcid.org/0000-0001-9215-9307"
  - family-names: Stallinga
    given-names: Sjoerd
    orcid: "https://orcid.org/0000-0003-4978-3272"
title: "Software for &#34;Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body&#34;"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/e0d424e4-5833-4729-a5a0-48ce94ed3f16.v1
license: Apache-2.0
date-released: 2025-10-01