cff-version: 1.2.0
abstract: "<p>The research objective was to quantify at the single-molecule level the motion of fully in vitro reconstituted CMG, the eukaryotic replicative helicase. </p>
<p><br></p>
<p>This was achieved by using a combination of optical tweezers and confocal fluorescence microscopy. The position along DNA molecules held in an optical trap of fluorescently labeled CMG was tracked over time, and different motion parameters were studied (e.g. diffusion constants and velocities) under different biochemical conditions.</p>"
authors:
  - family-names: Dekker
    given-names: Nynke
  - family-names: Ramirez Montero
    given-names: Daniel
    orcid: "https://orcid.org/0000-0003-3198-9811"
  - family-names: van Veen
    given-names: Edo
  - family-names: Sánchez
    given-names: Humberto
    orcid: "https://orcid.org/0000-0003-2137-1233"
  - family-names: van Laar
    given-names: Theo
  - family-names: Solano Hermosilla
    given-names: Belen
  - family-names: FX Diffley
    given-names: John
title: "Data underlying the publication: “Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation”"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/19948253.v1
license: CC BY 4.0
date-released: 2023-02-03