This study explored how strain anisotropy affects mechanosensitive Notch signaling and the resulting phenotype of vascular smooth muscle cells (VSMCs). We applied cyclic strains within the physiological range to compare the effects of equibiaxial and uniaxial loading on Notch activity and phenotypic changes in both synthetic and contractile VSMCs. By assessing three deformation parameters, we aimed to identify the main strain feature influencing these responses. Our findings indicate that VSMCs react similarly to both strain types, suggesting that strain anisotropy may not have a significant impact on Notch signaling and phenotype modulation.
This dataset contains the videos and data for strain analysis, immunofluorescence staining images, and real-time polymerase chain reaction data.
" authors: - family-names: Karakaya given-names: Cansu orcid: "https://orcid.org/0000-0002-2370-1868" - family-names: C. van Turnhout given-names: Mark - family-names: van den Hurk given-names: Eva A.N. orcid: "https://orcid.org/0009-0006-0039-3280" - family-names: Bouten given-names: Carlijn V.C. - family-names: Sahlgren given-names: Cecilia M. - family-names: Loerakker given-names: Sandra title: "Data underlying the publication "Equibiaxial and uniaxial cyclic strain similarly affect Notch signaling and vascular smooth muscle cell phenotype in 2D"" keywords: version: 1 identifiers: - type: doi value: 10.4121/6964ba10-be3a-4673-ba90-fb554ec751dd.v1 license: CC BY 4.0 date-released: 2025-05-20