Data underlying the study on Engineering class-B vitamin biosynthesis in Saccharomyces cerevisiae

doi:10.4121/17185607.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/17185607
Datacite citation style:
Jean-Marc Daran; Wrońska, Anna (2022): Data underlying the study on Engineering class-B vitamin biosynthesis in Saccharomyces cerevisiae. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/17185607.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite
Dataset

Saccharomyces cerevisiae is bradytroph for class B vitamins, it means that yeast cells exhibit slower growth in the absence of an external source of these metabolites. Alleviating these nutritional requirements for optimal growth performance would represent a valuable phenotypic characteristic for industrial strains since this would result in cheaper processes that would also be less susceptible to contaminations. In the present study, suboptimal growth of S. cerevisiae in absence of either pantothenic acid, para-aminobenzoic acid (pABA), pyridoxine, inositol and biotin were corrected by single or double gene overexpression of native FMS1, ABZ1/ABZ2, SNZ1/SNO1, INO1 and the Cyberlindnera fabianii BIO1, respectively. Several strategies were attempted to improve growth of S. cerevisiae CEN.PK113-7D in absence of thiamine, revealing that overexpression of THI4 and THI4/THI5 was able to improve growth up to 83% of the maximum specific growth rate of the reference CEN.PK113-7D in medium including all vitamins. Although the initial aim of this study was to combine all identified mutations in a single strain, the engineered strain IMX2210 only harboured genes to correct biotin, pABA, pantothenate and inositol bradotrophies. Firstly, this strain was fast-growing at a maximum specific growth rate of 0.28 ± 0.01 h-1 in medium devoid of all vitamins. Secondly, this strain exhibited physiological variables in aerobic glucose limited chemostat cultures at a dilution rate of 0.1 h-1 in absence of vitamins similar to that of the reference strain CEN.PK113-7D grown in the same conditions but in a fully supplemented complete medium. These physiological similarities were further emphasized by the limited differences observed in comparative transcriptome analysis from the chemostat culture grown cells that were essentially affecting genes of the class B vitamins biosynthetic pathways. This work paves the way towards construction of the first fast growing vitamin-independent S. cerevisiae strain.

history
  • 2022-12-23 first online, published, posted
publisher
4TU.ResearchData
format
Data in Excel files sequencing raw files are provided as fasq files
funding
  • European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie action PAcMEN (grant agreement no. 722287)
  • Predictive and Accelerated Metabolic Engineering Network (grant code 722287) [more info...] European Commission
organizations
TU Delft, Faculty Applied Sciences, Department of Biotechnology

DATA

files (12)