Population growth rate data and scripts concerning the publication:"Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast"experiments

DOI:10.4121/67e56fe8-6b54-446b-aaac-cd2e245ee066.v2
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/67e56fe8-6b54-446b-aaac-cd2e245ee066
Datacite citation style:
Iñigo De La Cruz, Leila; Dalmaan, Werner; Laan, Liedewij (2025): Population growth rate data and scripts concerning the publication:"Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast"experiments. Version 2. 4TU.ResearchData. dataset. https://doi.org/10.4121/67e56fe8-6b54-446b-aaac-cd2e245ee066.v2
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Dataset

choose version:
version 2 - 2025-02-11 (latest)
version 1 - 2023-07-19

The data entails raw and processed data from population growth rates experiments using different yeast mutants. Moreover, it contains the required scripts to process

the data and reproduce Fig3 and Supplemental Figures 4 and 5 from the paper "Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast" published in Nature Communications.

The experiments investigated the role of Cdc42 copy number in the polarization efficiency of different relevant mutants containing knockout mutations in Bem1 and Bem3 genes. The Cdc42 copy number was controlled using a galactose promoter.

Initially the growth measurements were taken using a sfGFP tag fussed in Cdc42 (data shown in Fig3). Therefore, we did control experiments, shown in the Supplemental material, to check that the sfGFP tag was not influencing the results we observed previously (Supp Fig 4 and Supp Fig 5).




History

  • 2023-07-19 first online
  • 2025-02-11 published, posted

Publisher

4TU.ResearchData

Format

data/.xlsx scripts/.mat scripts/.py

Organizations

Bionanoscience Department, Kavli Institute, Delft University of Technology

DATA

Files (1)