Simulated data for paper "Using splines for point spread function calibration at non-uniform depths in localization microscopy" (dataset)

Simulated data for paper "Using splines for point spread function calibration at non-uniform depths in localization microscopy"

doi:10.4121/5f75fadc-8f80-4e27-a049-1137031b19c0.v1

The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/5f75fadc-8f80-4e27-a049-1137031b19c0

Datacite citation style:

Korovin, Serafim; Kalisvaart, Dylan; Shih-te Hung; Cnossen, Jelmer; de Visser, Coen et. al. (2024): Simulated data for paper "Using splines for point spread function calibration at non-uniform depths in localization microscopy". Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/5f75fadc-8f80-4e27-a049-1137031b19c0.v1

Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

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keywords

Point spread function, Single-Molecule Localization Microscopy (SMLM), SMLM, Spline function, Super-resolution microscopy

licence

CC BY 4.0

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by Serafim Korovin , Dylan Kalisvaart , Shih-te Hung , Jelmer Cnossen , Coen de Visser , Carlas S. Smith

Supplementary example data for the paper "Using splines for point spread function calibration at non-uniform depths in localization microscopy".

The dataset consists of ".mat" files containing PSF function images or cspline models. There are three types of files in the dataset (XXXX refers to the calibration depth. ):

"astig_PSF_base_sphXXXX.mat" - Simulated astigmatic PSF function image z-stack with pure PSF functions (intensity sums to 1, no background, no shot noise)
"astig_PSF_diffspline_dpXXXX.mat" - Simulated astigmatic diffspline PSF model coefficients
"astig_smooth_sphXXXX_3Dcorr.mat" - Simulated astigmatic cspline PSF model coefficients

The data was generated with the code provided in open access on GitHub at https://github.com/qnano/diffspline. The data is also available in open access on SurfDrive at https://surfdrive.surf.nl/files/index.php/s/2oxletO4GkLAWbg.

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