The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/5b604dd5-5498-46aa-b437-775e957f93e3

Hu, Yimin; Schwab, Samuel; Qiu, Kaiyu; Zhang, Yunsen; Bär, Kerstin et. al. (2025): Data underlying the publication: DNA Wrapping by a Tetrameric Bacterial Histone. Version 2. 4TU.ResearchData. dataset. https://doi.org/10.4121/5b604dd5-5498-46aa-b437-775e957f93e3.v2

Version 1 - 2025-05-09

Categories

• Microbiology
• Evolutionary Biology
• Biochemistry and Cell Biology
• Biological Sciences

Keywords

Bacterial chromatin DNA wrapping Histones HLp Leptospira perolatii Nucleoid Associated Proteins

Licence

CC BY 4.0

Interoperability

• RO-Crate Metadata

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by Yimin Hu , Samuel Schwab , Kaiyu Qiu , Yunsen Zhang, Kerstin Bär, Heidi Reichle, Aurora Panzera, Andrei N. Lupas , Marcus D. Hartmann , Remus T. Dame , Vikram Alva , Birte Hernandez Alvarez

The research objective was to study how histone HLp from Leptospira perolatii binds and structures DNA. We have studied this with light microscopy, electrophoretic mobility shift assays, micrococcal nuclease assays, DNA topology assays, ligase-mediated circularisation, tethered particle motion (TPM), X-ray crystallography, and molecular dynamics (MD) simulations. This dataset contains the raw data of obtained root mean squared displacement (TPM).

History

• 2025-05-09 first online

• 2025-05-13 published, posted

Publisher

4TU.ResearchData

Format

.xlsx and related README .text files

Associated peer-reviewed publication

DNA Wrapping by a Tetrameric Bacterial Histone

Funding

• NWO grant OCENW.GROOT.2019.012 (grant code OCENW.GROOT.2019.012) NWO

Organizations

Department of Protein Evolution, Max Planck Institute for Biology Tübingen;
BioOptics Facility, Max Planck Institute for Biology Tübingen;
Interfaculty Institute of Biochemistry, University of Tübingen;
Theoretical and Computational Biophysics Group, Beckman Institute for Advanced Science and Technology, Center of Biophysics and Quantitative Biology, University of Illinois Urbana-Champaign;
Leiden Institute of Chemistry, Leiden University;
Centre for Microbial Cell Biology, Leiden University;
Centre for Interdisciplinary Genome Research, Leiden University