Data underlying the study on Engineering Saccharomyces cerevisiae for fast vitamin-independent aerobic growth

doi:10.4121/4f77d092-450d-4354-ac61-50241f2c7e95.v1
The doi above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
doi: 10.4121/4f77d092-450d-4354-ac61-50241f2c7e95
Datacite citation style:
Jean-Marc Daran; Wrońska, Anna; Perli, T. (Thomas); van den Broek, Marcel (2023): Data underlying the study on Engineering Saccharomyces cerevisiae for fast vitamin-independent aerobic growth. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/4f77d092-450d-4354-ac61-50241f2c7e95.v1
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Dataset

Chemically defined media for cultivation of Saccharomyces cerevisiae strains are commonly supplemented with a mixture of multiple Class-B vitamins, whose omission leads to strongly reduced growth rates. Fast growth without vitamin supplementation is interesting for industrial applications, as it reduces costs and complexity of medium preparation and may decrease susceptibility to contamination by auxotrophic microbes. In this study, suboptimal growth rates of S. cerevisiae CEN.PK113-7D in the absence of pantothenic acid, para-aminobenzoic acid (pABA), pyridoxine, inositol and/or biotin were corrected by single or combined overexpression of ScFMS1, ScABZ1/ScABZ2, ScSNZ1/ScSNO1, ScINO1 and Cyberlindnera fabianii BIO1, respectively. Several strategies were explored to improve growth of S. cerevisiae CEN.PK113-7D in thiamine-free medium. Overexpression of ScTHI4 and/or ScTHI5 enabled thiamine-independent growth at 83% of the maximum specific growth rate of the reference strain in vitamin-supplemented medium. Combined overexpression of seven native S. cerevisiae genes and CfBIO1 enabled a maximum specific growth rate of 0.33 ± 0.01 h-1 in vitamin-free synthetic medium. This specific growth rate was only 18 % lower than that of a congenic reference strain in vitamin-supplemented medium. Physiological parameters of the engineered vitamin-independent strain in aerobic glucose-limited chemostat cultures (dilution rate 0.10 h-1) grown on vitamin-free synthetic medium were similar to those of similar cultures of the parental strain grown on vitamin-supplemented medium. Transcriptome analysis revealed only few differences in gene expression between these cultures, which primarily involved genes with roles in Class-B vitamin metabolism. These results pave the way for development of fast-growing vitamin-independent industrial strains of S. cerevisiae.

history
  • 2023-09-27 first online, published, posted
publisher
4TU.ResearchData
format
Numerical data are provided in Excel files
funding
  • European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie action PAcMEN (grant code No 722287) [more info...] European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie actio
  • Advanced Grant of the European Research Council (grant code # 694633) [more info...] European Research Council
organizations
Delft University of Technology, Faculty of Applied Sciences, Department of Biotechnology

DATA

files (5)