Data underlying the publication: Nitrous Oxide as a Major Product of Anoxic Bioanode Ammonium Oxidation: Pathway Insights and Implications

DOI:10.4121/4b6b112f-d2ce-4f8c-867f-70bb651f27b5.v1
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/4b6b112f-d2ce-4f8c-867f-70bb651f27b5

Datacite citation style

Yan, Xiaofang; Yang, Zhenqiu; de Smit, Sanne; Liu, Dandan; Buisman, Cees et. al. (2025): Data underlying the publication: Nitrous Oxide as a Major Product of Anoxic Bioanode Ammonium Oxidation: Pathway Insights and Implications. Version 1. 4TU.ResearchData. dataset. https://doi.org/10.4121/4b6b112f-d2ce-4f8c-867f-70bb651f27b5.v1
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Anoxic ammonium (NH4+) oxidation at bioanodes offers a feasible alternative to conventional biological nitrogen removal (BNR), eliminating the need for aeration and enabling direct nitrogen gas (N2) production. However, the extent of nitrous oxide (N2O) emissions in this process remains unclear. This study examines N2O emissions during anoxic NH4+ oxidation at a polarized bioanode (+0.55 V vs Ag/AgCl), focusing on the roles of key intermediates—hydroxylamine (NH2OH) and nitrite (NO2-)—in N2O formation, and microbial functional groups involved in N2O production, aiming to elucidate the underlying N2O production pathways during bioanodic NH4+ oxidation. Results show that up to 40% of oxidized NH4+ was converted to N2O, while NO2- and NO3- remained at relatively low levels (2.1%–11.1%). NH2OH oxidation was identified as the dominant N2O production pathway, likely involving nitric oxide (NO) as an intermediate. NO2- reduction also contributed to N2O formation, albeit to a minor extent, while in situ N2O reduction—considered to be mediated by denitrifiers—offers potential for mitigating its emissions. In parallel, an electro-anammox-like pathway, which does not involve nitrifiers, facilitated direct NH4+ conversion to N2 without N2O formation. These findings highlight the potential for substantial N2O emissions from bioanode-based systems and underscore the importance of pathway control to mitigate greenhouse gas release.

History

  • 2025-07-08 first online, published, posted

Publisher

4TU.ResearchData

Format

excel/xlsx

Organizations

Environmental Technology, Wageningen University & Research;
Paqell B.V.

DATA

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