Data underlying the publication: Linking Metastatic Potential and Viscoelastic Properties of Breast Cancer Spheroids via Dynamic Compression and Relaxation in Microfluidics
doi: 10.4121/0af4ded1-d90f-405b-9a2e-482eae8a8a3a
The research objective is to investigate the relationship between the viscoelastic properties of breast cancer spheroids and their metastatic potential. The study employs a microfluidic device to subject spheroids to controlled dynamic compression, followed by monitoring their relaxation behavior.
The experiments were performed on a brightfield microscope, while the immunostaining images were taken on a confocal microscope. The images were extracted as .tif files and implemented in custom made MATLAB codes to analyse the spheroid compression and relaxation dynamics. Parameters like axial strain (for compression), deformation parameters (for relaxation), circularity were defined and fitted to physical models to extract viscoelastic parameters and relaxation timescales. The file include also the codes to generate the final figures included in the manuscript. A README file helps navigating the folders.
- 2025-01-09 first online, published, posted
- European Union's Horizon 2020 research and innovation program (grant code 819424) European Research Council (ERC)
- HORIZON EUROPE Marie Sklodowska-Curie Postdoctoral Fellowship 2022 (grant code 101111247)
DATA
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Paper - AdvHealthMat.zip -
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